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Cover The bacterial DNA translocase SpoIIIE strips proteins off the forespore chromosome during DNA transport across the septum during sporulation. Shown here are tiled fluorescent micrographs of wild-type sporulating Bacillus subtilis cells stained with a membrane dye (red), expressing either SpoIIIE-GFP (green) or a functional fusion of the β′ subunit of RNA polymerase to GFP (false-colored blue). During sporulation, SpoIIIE translocates 75% of the chromosome into the spore compartment. Images with green foci show SpoIIIE-GFP at the polar division plane. Images with blue show RNA polymerase associated with the chromosome. RNA polymerase and other DNA-binding proteins are stripped off during translocation, suggesting that much of the DNA arrives in the spore compartment naked, ready to receive and carry out the dictates of the developing spore. (For details, see Marquis et al., p. 1786.)